Optical filter sets for fluorescence imaging
Semrock has released four optical filter sets for fluorescence imaging that enable increased brightness or enhanced contrast when imaging labels fluorescein (FITC) and green fluorescent protein (GFP).
The new Brightline filter sets allow the user to either minimise photobleaching while increasing image contrast or receive a light boost for a brighter image, particularly in low-concentration applications such as single-molecule imaging.The GFP-1828-A and FITC-2024-A single-band filter sets offer a high contrast, while the GFP-4050-A and FITC-5050-A sets give the user high brightness among sets optimised for these fluorophores.
Semrock will continue to offer the Brightline GFP and FITC sets, offering a balance of brightness and contrast in a single three-filter set.The new filter sets extend the performance in both directions.The company's hard-coated filter manufacturing process ensures permanent performance with 'no burn-out' even under the intense illumination conditions.